Kaneda T, Nakajima A, Fujimoto K, Kobayashi T, Kiriyama S, Ebihara K, Innami T, Tsuji K, Tsuji E, Kinumaki T, Shimma H, Yoneyama S
J Nutr Sci Vitaminol (Tokyo). 1980;26(5):497-505. doi: 10.3177/jnsv.26.497.
In order to develop a simple and exact analytical method for cholesterol determination in foods by gas-liquid chromatography, several experiments were carried out in collaboration with several universities and institutes. For the extraction of lipids from foods, it was decided that chloroform-methanol (2:1) extraction was the most suitable procedure. Since pretreatment procedures using methods such as thin-layer chromatography and florisil column chromatography to purify the unsaponifiable matters reduced the recovery of cholesterol, and good results were obtained without applying the pretreatment, these procedures were concluded to be unnecessary. Gas chromatograms obtained with free sterol showed results similar to those of the trimethylsilyl ether derivative and acetate. 5-alpha-Cholestane is used as a good internal standard.
为了开发一种通过气液色谱法测定食品中胆固醇的简单准确的分析方法，与几所大学和研究所合作进行了多项实验。对于从食品中提取脂质，确定氯仿 - 甲醇（2:1）萃取是最合适的方法。由于使用薄层色谱法和弗罗里硅土柱色谱法等方法对不皂化物进行纯化的预处理程序会降低胆固醇的回收率，并且在不进行预处理的情况下也能获得良好的结果，因此得出结论这些程序是不必要的。用游离甾醇获得的气相色谱图显示的结果与三甲基硅醚衍生物和乙酸酯的结果相似。5-α-胆甾烷用作良好的内标。
