气相色谱法测定食品中的胆固醇及其他甾醇

Gas-liquid chromatographic determination of cholesterol and other sterols in foods.

作者信息

Sheppard A J, Newkirk D R, Hubbard W D, Osgood T

出版信息

J Assoc Off Anal Chem. 1977 Nov;60(6):1302-6.

Abstract

A gas-liquid chromatographic (GLC) method, using the butyryl esters of sterols, has been developed for the measurement of cholesterol, stigmasterol, sitosterol, and campesterol in foods. An immobile phase of 1% SE-30 coated on 100-120 mesh Gas-Chrom Q packed in a 6 inch X 4 mm id glass column operated at 255 degrees C was the most satisfactory of 7 column packings evaluated. Extraction with chloroform-methanol gave 98.7% recovery with a coefficient of variation of 1.8% for cholesterol added to a variety of foods. When cholesteryl palmitate was added to vegetable oil and the butyryl derivative was prepared, followed by GLC analysis, the recovery was 99.3% with a coefficient of variation of 0.9%. Amounts as low as 1 mg/100 g food can be detected with a precision of 2.5%. The results of the analysis of a variety of foods for cholesterol, campesterol, sitosterol, and stigmasterol are given.

摘要

已开发出一种气相色谱（GLC）方法，该方法使用甾醇的丁酰酯来测定食品中的胆固醇、豆甾醇、谷甾醇和菜油甾醇。在一根6英寸×4毫米内径的玻璃柱中填充100 - 120目气相色谱Q并涂覆1%的SE - 30作为固定相，在255℃下操作，这是所评估的7种柱填料中最令人满意的。用氯仿 - 甲醇萃取时，添加到各种食品中的胆固醇回收率为98.7%，变异系数为1.8%。当将棕榈酸胆固醇酯添加到植物油中并制备丁酰衍生物，随后进行气相色谱分析时，回收率为99.3%，变异系数为0.9%。最低可检测到1毫克/100克食品，精密度为2.5%。给出了对各种食品中胆固醇、菜油甾醇、谷甾醇和豆甾醇的分析结果。