Soloski M J, Holowczak J A
J Virol. 1981 Feb;37(2):770-83. doi: 10.1128/JVI.37.2.770-783.1981.
Treatment of vaccinia virions with 1% sodium dodecyl sulfate in the absence of reducing agents resulted in the release of subviral particles termed "subnucleoids," which contained viral DNA in combination with four polypeptides with molecular weights of 90,000, 68,000, 58,000 and 10,000. Biochemical and electron microscopic studies showed that viral DNA in combination with these polypeptides was maintained in a superhelical configuration. When subnucleoids were "fixed" with glutaraldehyde and formaldehyde and then examined by electron microscopy, spherical particles were observed, in which the supercoiled DNA was folded into globular structures that were 20 to 60 nm in diameter and were interconnected by DNA-protein fibers resembling the nucleosome structures described for eucaryotic chromatin.
在没有还原剂的情况下，用1%十二烷基硫酸钠处理痘苗病毒粒子，导致释放出称为“亚核样体”的亚病毒颗粒，其含有与分子量分别为90,000、68,000、58,000和10,000的四种多肽结合的病毒DNA。生化和电子显微镜研究表明，与这些多肽结合的病毒DNA保持超螺旋结构。当亚核样体用戊二醛和甲醛“固定”，然后通过电子显微镜检查时，观察到球形颗粒，其中超螺旋DNA折叠成直径为20至60纳米的球状结构，并通过类似于真核染色质中描述的核小体结构的DNA-蛋白质纤维相互连接。
