Bennell M A, Watson D L
Microbiol Immunol. 1980;24(9):871-8. doi: 10.1111/j.1348-0421.1980.tb02891.x.
The purpose of these studies was to determine the proportion of each immunoglobulin class/subclass in blood and colostrum of the pig and sheep, which would bind to staphylococcal Protein A. The concentrations of porcine IgG, IgM, and IgA were determined for serum and colostral whey from five sows. Similar measurements were made on two fractions produce by elution of the sample through a Protein A-Sepharose column: fraction 1, immunoglobulins which did not bind to Protein A, and fraction 2, immunoglobulins which bound to Protein A. The concentrations of ovine IgG1, IgG2, IgM, and IgA were measured for serum and colostral whey from six ewes, and again similar measurements were made after elution of each ovine sample through Protein A-Sepharose. All classes/subclasses of porcine and ovine serum and colostral immunoglobulins bound to Protein A to some extent. More than 90% of IgG from both porcine colostral whey and serum bound to Protein A. Ovine IgG1 from most ewes possessed a low affinity for Protein A whereas ovine IgG2 generally possessed a high affinity; 100% of the IgG2 in ovine colostral whey samples bound to Protein A. There was remarkable variation between individuals in the binding capacity of porcine IgM and each of the ovine immunoglobulins. For the ovine samples, in particular there were distinct differences between Protein A binding capacity of serum and colostral immunoglobulins of the same class/subclass.
这些研究的目的是确定猪和绵羊血液及初乳中能与葡萄球菌蛋白A结合的每种免疫球蛋白类别/亚类的比例。测定了五头母猪血清和初乳清中的猪IgG、IgM和IgA浓度。对通过蛋白A-琼脂糖凝胶柱洗脱样品得到的两个组分进行了类似测量：组分1为未与蛋白A结合的免疫球蛋白，组分2为与蛋白A结合的免疫球蛋白。测定了六头母羊血清和初乳清中的绵羊IgG1、IgG2、IgM和IgA浓度，并且在每个绵羊样品通过蛋白A-琼脂糖凝胶柱洗脱后再次进行了类似测量。猪和绵羊血清及初乳中的所有免疫球蛋白类别/亚类在一定程度上都能与蛋白A结合。猪初乳清和血清中的IgG超过90%能与蛋白A结合。大多数母羊的绵羊IgG1对蛋白A的亲和力较低，而绵羊IgG2通常具有较高的亲和力；绵羊初乳清样品中的IgG2 100%能与蛋白A结合。猪IgM以及每种绵羊免疫球蛋白的结合能力在个体之间存在显著差异。对于绵羊样品，同一类别/亚类的血清和初乳免疫球蛋白在蛋白A结合能力方面尤其存在明显差异。
