Wender D F, Thulin G E, Smith G J, Warshaw J B
Pediatr Res. 1981 Mar;15(3):262-8. doi: 10.1203/00006450-198103000-00013.
The effects of parenteral vitamin E treatment on aspects of the pulmonary biochemical and morphologic response to 100% oxygen were studied in newborn rabbits manifesting chemical evidence of vitamin E deficiency. Pups treated with 2 mg/100 g body weight increased serum vitamin E levels from 0.39 to 2.17 mg/dl by 72 hr and lung tissue vitamin E content from 3.52 to 17 mg/mg wet weight of lung. In vitro lipid peroxidation in lung homoginates of animals in 100% oxygen for 72 hr was inhibited by approximately 80% in animals receiving 100% oxygen plus vitamin E. Hyperoxia-induced increases in the pulmonary antioxidant enzymes, superoxide dismutase, glutathione peroxidase, and glutathione reductase were diminished by vitamin E administration. Lungs from vitamin E-treated animals did not show the early lung epithelial injury seen in animals exposed to 100% oxygen but not treated with vitamin E. Mophometric analysis of lungs of animals in room air for 72 hr showed 81.6% of lung to be normal as compared with 43.3% normal lung in the group maintained in 100% oxygen for 72 hr. In the group treated with oxygen plus vitamin E, the lungs were similar to room air controls (82.6% normal). This study thus provides further evidence for a direct antioxident affect of vitamin E in lung.
在表现出维生素E缺乏化学证据的新生兔中，研究了胃肠外给予维生素E治疗对肺部生化和形态学对100%氧气反应方面的影响。用2mg/100g体重治疗的幼崽在72小时内使血清维生素E水平从0.39mg/dl升至2.17mg/dl，肺组织维生素E含量从3.52mg/mg肺湿重增至17mg/mg肺湿重。在接受100%氧气加维生素E的动物中，100%氧气环境下72小时的动物肺匀浆中的体外脂质过氧化受到约80%的抑制。维生素E给药可减轻高氧诱导的肺部抗氧化酶超氧化物歧化酶、谷胱甘肽过氧化物酶和谷胱甘肽还原酶的增加。维生素E治疗动物的肺未显示出暴露于100%氧气但未接受维生素E治疗的动物中早期出现的肺上皮损伤。对在室内空气中放置72小时的动物的肺进行形态计量分析显示，81.6%的肺正常，而在100%氧气环境下维持72小时的组中正常肺为43.3%。在氧气加维生素E治疗组中，肺与室内空气对照组相似（82.6%正常）。因此，本研究为维生素E在肺中的直接抗氧化作用提供了进一步证据。
