Wispé J R, Knight M, Roberts R J
Pediatr Res. 1986 Jun;20(6):505-10. doi: 10.1203/00006450-198606000-00004.
The extent of in vivo lipid peroxidation and the in vivo antioxidant effects of alpha-tocopherol and alpha-tocopheryl acetate were studied in newborn rabbits exposed to one of two oxidant stresses: hyperoxia (FIO2 greater than 0.9) or parenteral lipid emulsion infusion. Lipid peroxidation was monitored by measurement of expired ethane and pentane, tissue thiobarbituric acid (TBA) reactants, and tissue lipid peroxides. Seventy-two h of hyperoxia did not increase any of the parameters of lipid peroxidation although mortality was higher in oxygen exposed animals. alpha-Tocopherol (100 mg/kg, intravenous) lowered expired hydrocarbons and tissue TBA reactants, but raised liver lipid peroxides in both air and hyperoxia exposed pups. Infusion of soybean oil emulsion increased production of ethane and pentane, liver TBA reactants, and lung lipid peroxides. Both alpha-tocopherol and alpha-tocopheryl acetate prevented the soybean oil emulsion induced increase in volatile hydrocarbons. alpha-Tocopherol (100 mg/kg, intravenous) administration also prevented the increase in liver TBA reactants and lung lipid peroxides. In identically treated animals, alpha-tocopheryl acetate administration decreased liver TBA reactants but had no effect on lung lipid peroxides. We conclude that alpha-tocopherol reduces lipid peroxidation in newborn rabbits including animals exposed to hyperoxia or infused with lipid emulsions. alpha-Tocopheryl acetate results in lower tissue alpha-tocopherol concentrations and is less effective as an antioxidant in lipid emulsion infused rabbits.
在暴露于两种氧化应激之一的新生兔中，研究了体内脂质过氧化程度以及α-生育酚和α-生育酚醋酸酯的体内抗氧化作用：高氧（FIO2大于0.9）或胃肠外脂质乳剂输注。通过测量呼出的乙烷和戊烷、组织硫代巴比妥酸（TBA）反应物以及组织脂质过氧化物来监测脂质过氧化。72小时的高氧并未增加脂质过氧化的任何参数，尽管暴露于氧气的动物死亡率更高。α-生育酚（100mg/kg，静脉注射）降低了呼出的碳氢化合物和组织TBA反应物，但在空气和高氧暴露的幼崽中均提高了肝脏脂质过氧化物。输注大豆油乳剂增加了乙烷和戊烷的产生、肝脏TBA反应物以及肺脂质过氧化物。α-生育酚和α-生育酚醋酸酯均阻止了大豆油乳剂诱导的挥发性碳氢化合物增加。α-生育酚（100mg/kg，静脉注射）给药也阻止了肝脏TBA反应物和肺脂质过氧化物的增加。在相同处理的动物中，α-生育酚醋酸酯给药降低了肝脏TBA反应物，但对肺脂质过氧化物没有影响。我们得出结论，α-生育酚可降低新生兔包括暴露于高氧或输注脂质乳剂的动物的脂质过氧化。α-生育酚醋酸酯导致组织α-生育酚浓度降低，并且在输注脂质乳剂的兔中作为抗氧化剂效果较差。
