Benveniste J
Nouv Presse Med. 1981 Jan 24;10(3):165-9.
The author has designed a human basophil degranulation test based on a one-step method of basophil staining after exposure to a specific allergen. This very simple test is now commercially available as a ready-to-use kit. It explores allergic sensitivity at cellular level, probably through IgE-dependent sensitization of the basophil. It correlates positively with histamine release and the radio-allergo-sorbent test (RAST) and detects sensitivity to common allergens as well as to foods and chemicals, including drugs. In its present form, the test provides a simple, inexpensive and reliable means of diagnosing allergic diseases in vitro but does not lend itself to mass investigations. It has recently been developed further and can now be performed on the automatic Hemalog D Technicon machine.
作者基于一种在接触特定过敏原后对嗜碱性粒细胞进行一步染色的方法设计了一种人嗜碱性粒细胞脱颗粒试验。这种非常简单的试验现在作为一种即用型试剂盒在市场上销售。它可能通过嗜碱性粒细胞的IgE依赖性致敏作用，在细胞水平上探究过敏敏感性。它与组胺释放及放射变应原吸附试验（RAST）呈正相关，可检测对常见过敏原以及食物和化学物质（包括药物）的敏感性。就其目前的形式而言，该试验提供了一种简单、廉价且可靠的体外诊断过敏性疾病的方法，但不适用于大规模调查。最近它得到了进一步发展，现在可以在自动Hemalog D Technicon机器上进行检测。
