定量细胞化学装置：一种计算机化扫描显微密度计“颗粒计数器”。
Device for quantitative cytochemistry: a computerized scanning microdensitometer 'grain counter'.
作者信息
Markovic N S, Lipkin L E, Markovic O S, Wade M J
出版信息
J Histochem Cytochem. 1978 Oct;26(10):792-802. doi: 10.1177/26.10.722048.
A computerized scanning microdensitometer and autoradiographic grain counter was able to provide quantitative data on the cytochemical final reaction product formed within a single cell and also quantitate the kinetics of its formation. Optical density and area measurements were performed on hundreds of leukocytes from slides previously stained to demonstrate any one of a variety of reactions. These included cellular glycogen, lipids, peroxidase, esterases, alkaline phosphatase, and acid phosphatase. In addition to these slide studies, chamber studies with an adapted Dvorak-Stotler Chamber allowed the measurement of enzyme kinetics within single cells.
一台计算机化扫描显微密度计和放射自显影片颗粒计数器能够提供关于单个细胞内形成的细胞化学最终反应产物的定量数据，还能对其形成动力学进行定量分析。对先前染色以显示多种反应中任何一种反应的载玻片上的数百个白细胞进行光密度和面积测量。这些反应包括细胞糖原、脂质、过氧化物酶、酯酶、碱性磷酸酶和酸性磷酸酶。除了这些载玻片研究外，使用改良的德沃夏克 - 斯托特勒小室进行的小室研究能够测量单个细胞内的酶动力学。
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