Connor A M, Brimble P D, Choy P C
Prep Biochem. 1981;11(1):91-7. doi: 10.1080/00327488108068727.
Phosphatidylcholine with a labelled acyl group at 2 position was prepared enzymatically from lysophosphatidylcholine and labelled fatty acids. Under optimal conditions, over 90% of [5,6,8,9,11,12,14,15-3H] arachidonic acid, [9,10-3H] palmitic acids, [1-14C] linoleic acid or [9,10-3H] oleic acid was incorporated into the phosphatidylcholine, and the incorporation was quantitative up to 500 nmol of [1-14C] linoleic acid. Analysis of the labelled phosphatidylcholine formed indicated that radioactivity was incorporated exclusively at 2-acyl position.
在2位带有标记酰基的磷脂酰胆碱是由溶血磷脂酰胆碱和标记脂肪酸通过酶法制备的。在最佳条件下，超过90%的[5,6,8,9,11,12,14,15-³H]花生四烯酸、[9,10-³H]棕榈酸、[1-¹⁴C]亚油酸或[9,10-³H]油酸被掺入到磷脂酰胆碱中，对于高达500 nmol的[1-¹⁴C]亚油酸，掺入是定量的。对形成的标记磷脂酰胆碱的分析表明，放射性仅在2-酰基位置掺入。
