Schulze G, Jung K, Kunze D, Egger E
Acta Biol Med Ger. 1976;35(1):1-5.
The preparation of PC with the 14C-fatty acids palmitic, stearic, oleic and linoleic acid at OH-group in position 2 is described. The starting material is egg yolk lecithin. By the attack of snake venom phospholiphase lyso-PC is produced which is reacylated by the appropriate fatty acid anhydride. In comparison with the methods published up to now this preparation has the advantage of higher yields and greater simplicity. By means of in vivo synthesis it is impossible to get PC species with only one fatty acid in a defined position. Working with radioactive fatty acids the specific radioactivity can be adapted to the requirements. The procedure can be made on a semi-technical scale.
描述了用14C标记的棕榈酸、硬脂酸、油酸和亚油酸在2位的羟基处制备磷脂酰胆碱（PC）的方法。起始原料是蛋黄卵磷脂。通过蛇毒磷脂酶的作用产生溶血磷脂酰胆碱，然后用适当的脂肪酸酐进行再酰化。与目前已发表的方法相比，该制备方法具有产率更高、操作更简便的优点。通过体内合成不可能得到在特定位置仅含一种脂肪酸的PC种类。使用放射性脂肪酸时，比放射性可根据需要进行调整。该方法可在半工业规模上进行。
