Peterson D A, Gerrard J M, Peller J, Rao G H, White J G
Prostaglandins Med. 1981 Jan;6(1):91-9. doi: 10.1016/s0161-4630(81)80012-2.
To probe the interaction of zinc with polyunsaturated fatty acids we have studied the effect of zinc on the cooxygenation of ferrous iron and arachidonic acid. Zinc inhibited the process of cooxygenation in a concentration dependent fashion. Further evaluation of the interaction of zinc and arachidonic acid gave spectroscopic evidence that zinc, oxygen and arachidonic acid can form an unstable hydroperoxide-like complex similar to that postulated earlier for iron, oxygen and arachidonic acid. However, in the case of zinc the complex will not proceed further to form stable peroxides and the unstable complex falls apart to give zinc and arachidonic acid intact. The findings have implications for the role of zinc in enzyme reactions and for antioxidant reactions of zinc within the cell. The influence of zinc on platelet aggregation was also evaluated. Zinc was found to inhibit cell-cell aggregation. However, in contrast to the known ability of zinc to inhibit prostaglandin synthesis in a broken cell preparation, zinc did not inhibit prostaglandin or thromboxane synthesis in the intact platelet. Inhibition of platelet aggregation by zinc must result from some other action of this cation.
为了探究锌与多不饱和脂肪酸之间的相互作用，我们研究了锌对亚铁离子和花生四烯酸共氧化作用的影响。锌以浓度依赖的方式抑制了共氧化过程。对锌与花生四烯酸相互作用的进一步评估提供了光谱学证据，表明锌、氧和花生四烯酸能够形成一种不稳定的类氢过氧化物复合物，类似于之前推测的铁、氧和花生四烯酸形成的复合物。然而，就锌而言，该复合物不会进一步形成稳定的过氧化物，不稳定的复合物会分解，使锌和花生四烯酸保持完整。这些发现对锌在酶反应中的作用以及细胞内锌的抗氧化反应具有启示意义。我们还评估了锌对血小板聚集的影响。发现锌能抑制细胞间聚集。然而，与锌在破碎细胞制剂中抑制前列腺素合成的已知能力相反，锌在完整血小板中并不抑制前列腺素或血栓素的合成。锌对血小板聚集的抑制作用一定是由该阳离子的其他某种作用引起的。
