Effect of gluconeogenic substrates on ketogenesis in isolated rat hepatocytes. I. Effect of glycerol.

The effect of glycerol on the rate of free fatty acids in isolated hepatocytes from starved rats has been studied. 5 mM glycerol inhibited the ketone bodies production from endogenous substrates, acetate, octanoate or oleate. The incorporation of radioactivity into ketone bodies from acetate, octanoate or oleate was also inhibited by glycerol. The oxidation of radioactive fatty acids to CO2 was not significantly changed by glycerol. However, the incorporation of acetate, octanoate or oleate carbons into liver lipids were increased by glycerol.