De Bandt J P, Cynober L, Rey C, Coudray-Lucas C, Lim S K, Poupon R, Giboudeau J
Laboratoire de Biochimie A, Hôpital Saint-Antoine, Paris.
Gastroenterol Clin Biol. 1992;16(1):46-9.
Large disparity in experimental conditions concerning the use of isolated perfused liver led us to investigate the effects of two free-fatty acids on amino acid metabolism in this model. Oleate uptake was 210 +/- 46 nmol/min.g and octanoate uptake 550 +/- 60 nmol/min.g. Fatty acid utilization led to an increase in ketogenesis, enhanced by octanoate in accordance with its known availability for beta-oxidation. In our experiments neither oleate nor octanoate modified hepatic amino acid exchange and metabolism as assessed by the measurements of amino acids fluxes and glucose and urea production.
在使用离体灌注肝脏的实验条件方面存在很大差异，这促使我们研究两种游离脂肪酸对该模型中氨基酸代谢的影响。油酸摄取量为210±46 nmol/分钟·克，辛酸摄取量为550±60 nmol/分钟·克。脂肪酸的利用导致生酮作用增加，辛酸根据其已知的β氧化可用性增强了这种作用。在我们的实验中，通过测量氨基酸通量以及葡萄糖和尿素生成来评估，油酸和辛酸均未改变肝脏氨基酸交换和代谢。
