Sugar derivatives and liver mitochondria. V - Effect of 6-O-benzoyl-3-deoxy-3-fluoro-1,2-O-isopropylidene-alpha-D-glucofuranose.

Using conventional polarographic and mannometric techniques for assaying oxidative phosphorylation and oxygen uptake in isolated rat liver mitochondria, it was observed a decrease in ADP:O ratio and RC coefficient as a function of treatment with 6-O-benzoyl-3-deoxy-3-fluoro-1,2-O-isopropylidene-alpha-D-glucofuranose, using succinate and alpha-ketoglutarate as substrates. The enzymes activities of different segments of the respiratory chain, indicated an inhibition of NADH-oxidase activity as a result of treatment with 6-O-benzoyl-3-deoxy-fluoro-1,2-O-isopropylidene-alpha-D-glucofuranose. The oxidation of NADH-linked substrates by rat liver mitochondria was depressed by the compound used, while the succinate oxidation was increased.