Reeves R B
Respir Physiol. 1980 Dec;42(3):299-315. doi: 10.1016/0034-5687(80)90121-8.
A method for recording complete dynamic oxygen equilibrium curves (O2EC) from microliter samples of whole blood is described. The blood sample is compressed into a thin film between two 6-micron thick Teflon membranes in order to promote rapid gas exchange with gas volume surrounding the membranes. Oxygen tension of the gas volume around the blood film is charged at a controlled rate from zero to ca. 100 Torr by a specially designated gas-exchanging cuvette. Saturation of red cell hemoglobin in the film is measured by dual-wavelength spectrophotometry using Soret wavelengths of 430-453 nm. Gas volume oxygen tension is measured with a Teflon membrane covered oxygen cathode. Full saturation is secured by introducing an oxygen mixture whose PO2 exceeds 650 Torr. A single O2EC can be run in about 4 min at 37 degrees; multiple O2EC can be recorded from the same blood film. Each curve is run isocapnically at a preselected carbon dioxide tension. Blood film pH is calculated from the CO2 partial pressure and an independently determined buffer line. Data are presented to show that normal standard curve for man as determined with this blood-film method and those determined by other methods are equivalent.
本文描述了一种从全血微升样本中记录完整动态氧平衡曲线（O2EC）的方法。将血样压缩在两片6微米厚的聚四氟乙烯膜之间形成薄膜，以促进与膜周围气体体积的快速气体交换。通过专门设计的气体交换比色皿，将血膜周围气体体积的氧张力以可控速率从零增加到约100托。使用430 - 453纳米的索雷特波长，通过双波长分光光度法测量膜中红细胞血红蛋白的饱和度。用覆盖有聚四氟乙烯膜的氧阴极测量气体体积氧张力。通过引入PO2超过650托的氧气混合物来确保完全饱和。在37摄氏度下，一条O2EC大约可以在4分钟内完成测量；可以从同一血膜记录多条O2EC。每条曲线在预选的二氧化碳张力下等碳酸条件下运行。根据二氧化碳分压和独立测定的缓冲线计算血膜pH值。数据表明，用这种血膜法测定的正常人标准曲线与其他方法测定的标准曲线是等效的。
