Robb S M, Woods D R, Robb F T
J Gen Virol. 1978 Nov;41(2):265-72. doi: 10.1099/0022-1317-41-2-265.
The growth characteristics of alpha3a bacteriophage on stationary phase Achromobacter strain 14 are described. Phage alpha3a growth on stationary phase cells is characterized by a long and variable latent period of 6 to 9 h and an increased burst size of 710 p.f.u./cell as compared with 153 p.f.u./cell in exponential wild type cells. During the latent period the infected cells are very sensitive to changes in growth conditions and in particular, dilution. Pre-conditioning of the bacterial cells by allowing them to stand for 24 h after shaking for 3 days is an important aspect of the stationary phase phage growth system. Cells which have been allowed to stand retain the ability to be infected and to support phage growth for at least 16 days. Shaking cultures gradually lose the ability to support phage growth but the phage can persist in the host cell for 10 days until removal from shaking when the lytic cycle can proceed after allowing the cultures to stand.
描述了α3a噬菌体在静止期无色杆菌14菌株上的生长特性。α3a噬菌体在静止期细胞上的生长特点是潜伏期长且可变，为6至9小时，与指数生长期野生型细胞中每个细胞产生153个噬菌斑形成单位相比，爆发量增加至每个细胞710个噬菌斑形成单位。在潜伏期内，被感染的细胞对生长条件的变化，特别是稀释非常敏感。通过在振荡培养3天后让细菌细胞静置24小时来进行预处理，是静止期噬菌体生长系统的一个重要方面。静置过的细胞保留了被感染并支持噬菌体生长至少16天的能力。振荡培养的细胞逐渐失去支持噬菌体生长的能力，但噬菌体可在宿主细胞中持续存在10天，直到停止振荡，当培养物静置后裂解周期才能继续进行。
