Robb S M, Robb F T, Woods D R
J Gen Microbiol. 1980 Aug;119(2):405-12. doi: 10.1099/00221287-119-2-405.
Growth of phase alpha 3a on stationary phase Vibrio cultures requires micro-aerophilic conditions and is inhibited by aeration. Since pre-conditioning of the bacteria by allowing them to stand for 24 h after shaking for 3 d is an important aspect of the stationary phase phage growth system, various physiological and morphological characteristics of the stationary phase cells during the transition from shaking to standing were investigated. Shaken stationary phase cells were less viable and more sensitive to ultraviolet irradiation and heat than standing stationary phase cells. During pre-conditioning the small, non-flagellated cells present in shaken stationary phase cultures underwent morphological changes and became large, flagellated rods which resembled exponential phase cells. The transition of stationary phase cells from shaking to standing was associated with a marked increase in total RNA synthesis but a rapid and large decrease in total protein synthesis. Intracellular concentrations of ATP in shaken stationary phase cells were 53% lower than those in standing stationary phase cells. Studies on leucine uptake indicated that its transport was inhibited by isoleucine and that the major part (90%) of the total leucine uptake was due to a shared system for uptake of both amino acids. Shaken stationary phase cells transported less leucine than standing stationary phase cells. Inhibition of phage growth in aerated stationary phase cultures was not due to the prevention of phase absorption by shaking. It is suggested that the observed differences between shaken and standing stationary phase cells could be due to aeration affecting the template specificity of the Vibrio RNA polymerase.
α3a期在静止期弧菌培养物上的生长需要微需氧条件，且会被通气抑制。由于在3天振荡培养后让细菌静置24小时对其进行预处理是静止期噬菌体生长系统的一个重要方面，因此研究了从振荡到静置过程中静止期细胞的各种生理和形态特征。与静置的静止期细胞相比，振荡的静止期细胞活力较低，对紫外线照射和热更敏感。在预处理过程中，振荡的静止期培养物中存在的小的、无鞭毛的细胞发生形态变化，变成了类似指数期细胞的大的、有鞭毛的杆菌。静止期细胞从振荡到静置的转变与总RNA合成的显著增加相关，但总蛋白质合成迅速大幅下降。振荡的静止期细胞内ATP浓度比静置的静止期细胞低53%。对亮氨酸摄取的研究表明，其转运受到异亮氨酸的抑制，并且总亮氨酸摄取的主要部分（90%）归因于两种氨基酸摄取的共用系统。振荡的静止期细胞比静置的静止期细胞转运的亮氨酸更少。通气的静止期培养物中噬菌体生长的抑制不是由于振荡阻止了噬菌体吸附。有人认为，振荡和静置的静止期细胞之间观察到的差异可能是由于通气影响了弧菌RNA聚合酶的模板特异性。
