Cameron D F, Markwald R R
Am J Anat. 1981 Mar;160(3):343-58. doi: 10.1002/aja.1001600310.
Sertoli cells were harvested from sexually mature rats and maintained in vitro for up to ten days (Sertoli cell-enriched cultures) or co-cultured with rat peritubular fibroblasts. Cultures were examined by differential light microscopy and by scanning or transmission electron microscopy. The presence of peritubular fibroblasts in co-culture greatly enhanced plating efficiency and viability of adult Sertoli cells. Sertoli cell aggregates preferentially adhered to peritubular cells, and by ten days had flattened and spread across these cells. Sertoli cells retained their characteristic ultrastructural features. Early in co-culture a collagen-like extracellular material was seen bridging Sertoli and peritubular cells, and its appearance was coincident with the presence of swollen rough endoplasmic reticulum in peritubular cells. Interperitubular cell spaces became engorged with a fibrillar material morphologically similar to the basal lamina of the seminiferous tubule wall. In the absence of peritubular cells, in culture medium "conditioned" by prior incubation with peritubular cells but not containing them, or when cultured with other fibroblastic cells, plating efficiency was low; Sertoli cells never flattened and cell ultrastructure progressively degenerated. The results indicated that peritubular cells support adult Sertoli cells in culture, possibly via extracellular material derived from peritubular cells, and suggest that Sertoli cells have an inductive effect on peritubular cell secretory activity.
从性成熟大鼠中获取支持细胞，并在体外培养长达十天（富含支持细胞的培养物），或将其与大鼠睾丸周成纤维细胞共培养。通过相差显微镜以及扫描或透射电子显微镜对培养物进行检查。共培养中睾丸周成纤维细胞的存在极大地提高了成年支持细胞的接种效率和活力。支持细胞聚集体优先黏附于睾丸周细胞，到十天时已变扁平并铺展在这些细胞上。支持细胞保留了其特征性超微结构特征。在共培养早期，可见一种类胶原细胞外物质连接支持细胞和睾丸周细胞，其出现与睾丸周细胞中肿胀的粗面内质网的存在相一致。睾丸周细胞间隙充满了一种形态上类似于生精小管壁基膜的纤维状物质。在没有睾丸周细胞的情况下，在先前与睾丸周细胞共同孵育但不含这些细胞的“条件”培养基中，或与其他成纤维细胞共培养时，接种效率很低；支持细胞从不扁平，细胞超微结构逐渐退化。结果表明，睾丸周细胞可能通过源自睾丸周细胞的细胞外物质在培养中支持成年支持细胞，并提示支持细胞对睾丸周细胞的分泌活性有诱导作用。
