Rao S S, Watt I A, Donaldson L A, Crocket A, Joffe S N
Am J Pathol. 1981 Apr;103(1):39-46.
This study was undertaken for the purpose of a serial investigation of the development and progression of the light-microscopic changes of acute pancreatitis and histologic criteria for evaluating pancreatitis. Acute pancreatitis, similar to that found in man, was induced in rats with the use of a closed duodenal loop technique (n = 36). Control rats underwent a laparotomy with mobilization of the duodenum (n = 12). Animals were killed every 2 hours for 24 hours, and a detailed and independent histologic evaluation was made of each. Focal acinar necrosis proceeding to a vasculitis appeared within 2--4 hours before the infiltration of inflammatory cells. Thereafter, the extent of acinar necrosis closely reflected the vasculitis with the later development of the acute inflammation. By the sixteenth hour, these changes were graded as moderate pancreatitis, and by 24 hours the process represented severe hemorrhagic pancreatitis. Vascular changes and acinar necrosis preceded the inflammatory cell infiltrate. The pancreatitis has been quantitated into minimal, moderate, or severe by assessing the severity of edema, acute inflammatory infiltrate, and changes in the vessels, ducts, and acini.
本研究旨在对急性胰腺炎光镜下变化的发展及进展情况以及评估胰腺炎的组织学标准进行系列研究。采用闭合十二指肠袢技术在大鼠中诱发与人相似的急性胰腺炎（n = 36）。对照大鼠接受十二指肠游离的剖腹手术（n = 12）。在24小时内每2小时处死一批动物，并对每只动物进行详细且独立的组织学评估。在炎症细胞浸润前2 - 4小时出现局灶性腺泡坏死并发展为血管炎。此后，腺泡坏死的程度与血管炎密切相关，随后急性炎症发展。到第16小时，这些变化被分级为中度胰腺炎，到24小时该过程表现为重度出血性胰腺炎。血管变化和腺泡坏死先于炎症细胞浸润出现。通过评估水肿、急性炎症浸润以及血管、导管和腺泡的变化严重程度，将胰腺炎定量分为轻度、中度或重度。
