Kaneene J M, Anderson R K, Muscoplat C C, Johnson D W
Am J Vet Res. 1979 Jul;40(7):999-1004.
Cell-mediated immune (CMI) responses in cattle vaccinated with Brucella abortus strain 19 vaccine during calfhood were studied by an in vitro lymphocyte stimulation assay. Cattle were grouped in six groups according to the age after vaccination, and CMI responses of these groups, as well as of individual animals, were compared. Lymphocytes were prepared from peripheral blood of these cattle by the Ficoll-diatrizoate technique. Lymphocytes were then cultured with B abortus-soluble antigen. Culture results were assayed for [3H]thymidine incorporation into DNA by liquid scintillation spectrometry. On a group basis, B abortus-soluble antigen induced lymphocyte stimulation responses in lymphocytes from all the groups, except the sixth group which contained animals that had been vaccinated the longest time (18 to 24 months before this experiment). Animals that had been vaccinated for 3 to 6 months had the highest lymphocyte stimulation response. Seroagglutination tests were conducted simultaneously with the lymphocyte stimulation test, but there was no apparent correlation between the concentrations of humoral antibodies and the CMI responses as measured by in vitro specific lymphocyte stimulation. The lymphocyte stimulation test exhibited significantly higher specificity (P less than 0.005) than the serologic tests.
通过体外淋巴细胞刺激试验，研究了犊牛期接种布鲁氏菌19号疫苗的牛的细胞介导免疫（CMI）反应。根据接种疫苗后的年龄将牛分为六组，并比较了这些组以及个体动物的CMI反应。采用聚蔗糖 - 泛影葡胺技术从这些牛的外周血中制备淋巴细胞。然后将淋巴细胞与流产布鲁氏菌可溶性抗原一起培养。通过液体闪烁光谱法测定培养结果中[3H]胸腺嘧啶核苷掺入DNA的情况。从组的角度来看，流产布鲁氏菌可溶性抗原在除第六组（该组动物接种疫苗时间最长，在本实验前18至24个月）之外的所有组的淋巴细胞中均诱导了淋巴细胞刺激反应。接种3至6个月的动物具有最高的淋巴细胞刺激反应。在进行淋巴细胞刺激试验的同时进行了血清凝集试验，但通过体外特异性淋巴细胞刺激测定的体液抗体浓度与CMI反应之间没有明显的相关性。淋巴细胞刺激试验的特异性显著高于血清学试验（P小于0.005）。
