Terasaki K, Spector E B, Hendrickson R, Cederbaum S D
Biochem Genet. 1980 Oct;18(9-10):829-41. doi: 10.1007/BF00500116.
Deficiency of arginase (E.C. 3.5.3.1), the fifth enzyme of the urea cycle, was found in the red blood cells (RBCs) of Macaca fascicularis monkeys (less than 0.2 micromol arginine cleaved/g Hb/min; normal equals 49.2). Liver biopsies were obtained from two of these monkeys and from one monkey with normal levels of RBC arginase activity. Arginase from both groups of animals required Mn2+ for maximal enzyme activity and demonstrated a pH optimum of 10.2 in vitro. The activity of arginase in the livers of all three monkeys was 1.1 millimol arginine cleaved per g protein per min. The apparent Km for arginine of arginase in the livers of the RBC-deficient monkeys was 7.4 and 5.9 mM and in the normal monkey was 6.9 mM. Similar patterns of heat denaturation was seen at 69 C without Mn2+ present and 79 C in the presence of 20mM Mn2+. No difference in mobility on either RBC-deficient or normal monkeys was found. In addition, liver arginase from all three monkeys reacted similarly with anti-human liver arginase antibody. Liver arginases in RBC-deficient and normal monkeys were identical by ten criteria. These studies do not distinguish among several hypotheses for the genetic determination of arginase in different organs of this species and of man.
在食蟹猴的红细胞中发现了精氨酸酶（E.C. 3.5.3.1）缺乏，精氨酸酶是尿素循环的第五种酶（低于0.2微摩尔精氨酸裂解/克血红蛋白/分钟；正常为49.2）。从其中两只猴子以及一只红细胞精氨酸酶活性水平正常的猴子身上获取了肝脏活检样本。两组动物的精氨酸酶都需要Mn2+才能达到最大酶活性，并且在体外表现出最适pH值为10.2。所有三只猴子肝脏中的精氨酸酶活性为每克蛋白质每分钟裂解1.1毫摩尔精氨酸。红细胞缺乏的猴子肝脏中精氨酸酶对精氨酸的表观Km值分别为7.4和5.9毫摩尔，正常猴子的为6.9毫摩尔。在不存在Mn2+的情况下，69℃时观察到相似的热变性模式；在存在20毫摩尔Mn2+的情况下，79℃时观察到相似的热变性模式。在红细胞缺乏的猴子和正常猴子中，迁移率均未发现差异。此外，所有三只猴子的肝脏精氨酸酶与抗人肝脏精氨酸酶抗体的反应相似。根据十个标准，红细胞缺乏的猴子和正常猴子的肝脏精氨酸酶是相同的。这些研究并未区分关于该物种和人类不同器官中精氨酸酶遗传决定的几种假设。
