James K, Davis S, Merriman J
Br J Cancer. 1981 Mar;43(3):294-304. doi: 10.1038/bjc.1981.47.
Using competitive double-antibody radioimmunoassays we have shown that immunoglobulin (especially IgA) can be recovered in pH 3.5, 0.12M acid citrate eluates of freshly excised CCH1 tumour-cell suspensions. Studies with 125I-labelled eluates indicate that such preparations exhibit a variable, but appreciable, degree of non-specific binding to unrelated syngeneic tumour and normal tissues. PAGE/SDS gel electrophoresis of the labelled eluates revealed the presence of a major non-immunoglobulin component of 33-36K dalton which could account in part for the non-specific binding observed. This component was also detected in similar eluates from cultured CCH1 tumour and in all other tumour-cell eluates examined to date. In contrast, preliminary data suggest that it is less prevalent in acid citrate eluates from normal tissue, with the exception of peritoneal-exudate cells. The possible origins, nature and significance of this non-immunoglobulin component are discussed.
使用竞争性双抗体放射免疫测定法，我们已证明免疫球蛋白（尤其是IgA）可在新鲜切除的CCH1肿瘤细胞悬液的pH 3.5、0.12M酸性柠檬酸盐洗脱液中回收。对125I标记洗脱液的研究表明，此类制剂与无关的同基因肿瘤和正常组织表现出不同程度但相当可观的非特异性结合。标记洗脱液的PAGE/SDS凝胶电泳显示存在一种33 - 36K道尔顿的主要非免疫球蛋白成分，这可能部分解释了所观察到的非特异性结合。在培养的CCH1肿瘤的类似洗脱液以及迄今检查的所有其他肿瘤细胞洗脱液中也检测到了该成分。相比之下，初步数据表明，除腹膜渗出细胞外，正常组织的酸性柠檬酸盐洗脱液中该成分不太常见。本文讨论了这种非免疫球蛋白成分的可能来源、性质和意义。
