Obrenovitch A, Sené C, Roche A C, Monsigny M, Visher P, Hughes R C
Biochimie. 1981 Mar;63(3):169-75. doi: 10.1016/s0300-9084(81)80190-3.
The cell surface glycoconjugates of Baby Hamster Kidney cells and of four ricin resistant variants were investigated by the use of 125I-substituted ricin (Ricinus communis toxin) which binds galactose residues, and by the use of fluorescein labelled lectins which bind N-acetylneuraminic acid and/or N-acetylglucosamine: Limulin (Limulus polyphemus agglutinin), wheat germ agglutinin (Triticum vulgare agglutinin) and succinylated wheat germ agglutinin. Striking differences in the number of lectin and/or ricin receptors were found between the cell surface of wild type cells and that of ricin resistant variants. The results are discussed on the basis of the main glycopeptide structure, and of the specificity of the sugar binding proteins used. The ricin resistance of variant cells is concomitant to modifications of the concentration of certain glycoconjugate structures which are accessible to the sugar binding proteins. Depending on the variants, N-asparaginyl glycopeptide types and/or O-glycosidic glycopeptide types are affected.
利用结合半乳糖残基的¹²⁵I标记蓖麻毒素（蓖麻毒蛋白），以及结合N-乙酰神经氨酸和/或N-乙酰葡糖胺的荧光素标记凝集素：鲎试剂（鲎凝集素）、麦胚凝集素（小麦凝集素）和琥珀酰化麦胚凝集素，对幼仓鼠肾细胞及其四种蓖麻毒素抗性变体的细胞表面糖缀合物进行了研究。野生型细胞和蓖麻毒素抗性变体的细胞表面在凝集素和/或蓖麻毒素受体数量上存在显著差异。根据主要糖肽结构以及所用糖结合蛋白的特异性对结果进行了讨论。变体细胞的蓖麻毒素抗性与某些糖结合蛋白可及的糖缀合物结构浓度的改变有关。根据变体的不同，N-天冬酰胺基糖肽类型和/或O-糖苷糖肽类型会受到影响。
