Rungger-Brändle E, Jamrich M, Bautz E K
Chromosoma. 1981;82(3):399-407. doi: 10.1007/BF00285765.
By means of indirect immunofluorescence microscopy, we have studied the distribution of RNA polymerase B, of the nucleosomal histones H2b, H3, and H4 and of histone H1, in nuclei of primary spermatocytes of Drosophila hydei. RNA polymerase B and histones, including H1, are found to be present on the loop structures of the Y chromosome. The nucleolus stains only for the histones, but not for RNA polymerase B. Various mutants deficient for some of the loops or altering their morphology, were used to identify the individual chromosomal segments. In growing spermatocytes of the genetic constitution X/0, autosomes and the chromosome X react strongly with antibodies against RNA polymerase B, but not with antibodies against histones. The results suggest that the autosomes, the chromosome X and the Y chromosomal loop structures, with the exception of the nucleolus, are transcribed mostly by RNA polymerase B.
通过间接免疫荧光显微镜技术，我们研究了海德果蝇初级精母细胞核中RNA聚合酶B、核小体组蛋白H2b、H3和H4以及组蛋白H1的分布情况。发现RNA聚合酶B和包括H1在内的组蛋白存在于Y染色体的环状结构上。核仁仅对组蛋白染色，而不对RNA聚合酶B染色。利用一些缺失部分环状结构或改变其形态的各种突变体来鉴定各个染色体片段。在遗传组成X/0的生长中的精母细胞中，常染色体和X染色体与抗RNA聚合酶B的抗体发生强烈反应，但与抗组蛋白的抗体不发生反应。结果表明，除核仁外，常染色体、X染色体和Y染色体的环状结构主要由RNA聚合酶B转录。
