通过间接免疫荧光研究RNA聚合酶在果蝇多线染色体上的分布。
Distribution of RNA polymerase on Drosophila polytene chromosomes as studied by indirect immunofluorescence.
作者信息
Plagens U, Greenleaf A L, Bautz E K
出版信息
Chromosoma. 1976 Dec 16;59(2):157-65. doi: 10.1007/BF00328484.
PMID:827428
Abstract
Using indirect immunofluorescence visualization techniques we investigated the in situ distribution of RNA polymerase B on Drosophila melanogaster polytene chromosomes. The enzyme was found at many sites distributed throughout the genome in a pattern clearly distinct from that observed for histone H1, but it was especially concentrated in puffs induced by heat shock.
摘要
我们使用间接免疫荧光可视化技术研究了RNA聚合酶B在黑腹果蝇多线染色体上的原位分布。发现该酶在整个基因组中分布于许多位点，其模式与组蛋白H1明显不同，但它特别集中在热休克诱导的胀泡中。
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