Mode A, Norstedt G, Simic B, Eneroth P, Gustafsson J A
Endocrinology. 1981 Jun;108(6):2103-8. doi: 10.1210/endo-108-6-2103.
The metabolism of 4-[4-14C]androstene-3,17-dione was studied in the microsomal fraction of rat livers after continuous administration of human GH (hGH) in Alzet osmotic minipumps under varying conditions. hGH caused a complete feminization of hepatic steroid metabolism (i.e. increased the 5 alpha-reductase and decreased the 6 beta- and 16 alpha-hydroxylase activities) in normal male rats when infused at a rate of 5 microgram/h for 7 days. Hypophysectomy and castration or adrenalectomy and thyroidectomy of male rats did not reduce the feminizing capacity of hGH, indicating that the adrenals and the thyroid gland are not involved in the mediation of the feminizing effect of hGH. The same dose (5 microgram/h for 7 days) of hGH was also able to refeminize the liver steroid metabolism in hypophysectomized-ovariectomized female rats. The effect of the homologous hormones, rat PRL and rat GH on hepatic steroid metabolism was also investigated. Either hormone was infused at a rate of 10 microgram/h for 7 days, a dose which was sufficient to increase the serum level of the hormone in hypophysectomized animals. No feminizing effect was seen after the administration of rat PRL, whereas rat GH caused a partial feminization of hepatic steroid metabolism in hypophysectomized male animals. It is concluded that GH or a hormone related to GH is involved in sexual differentiation of liver steroid metabolism.
在不同条件下，通过Alzet渗透微型泵持续给大鼠注射人生长激素（hGH）后，研究了4-[4-¹⁴C]雄甾烯-3,17-二酮在大鼠肝脏微粒体部分的代谢情况。当以5微克/小时的速率注射7天时，hGH可使正常雄性大鼠的肝脏类固醇代谢完全女性化（即增加5α-还原酶活性，降低6β-和16α-羟化酶活性）。雄性大鼠垂体切除和去势或肾上腺切除和甲状腺切除并未降低hGH的女性化能力，这表明肾上腺和甲状腺不参与hGH女性化作用的介导。相同剂量（5微克/小时，持续7天）的hGH也能够使垂体切除-卵巢切除的雌性大鼠的肝脏类固醇代谢重新女性化。还研究了同源激素大鼠催乳素（PRL）和大鼠生长激素（GH）对肝脏类固醇代谢的影响。两种激素均以10微克/小时的速率注射7天，该剂量足以提高垂体切除动物的激素血清水平。注射大鼠PRL后未观察到女性化作用，而大鼠GH可使垂体切除的雄性动物的肝脏类固醇代谢部分女性化。结论是GH或与GH相关的一种激素参与肝脏类固醇代谢的性别分化。
