Fontagné J, Adolphe M, Semichon M, Zizine L, Lechat P
Exp Hematol. 1980 Oct;8(9):1157-64.
The effect of indomethacin (6 mg/kg daily orally for 2, 3 or 4 days) on myelopoiesis was studied in mice by estimating 1) differential cell counts of bone marrow, 2) proliferation of the CFUC in presence of GM-CSF, 3) proliferative state of CFUC after tritiated thymidine suicide. After 4 days of treatment, a rise in the recognizable myeloid precursors, an increase of both the colony forming capacity and the number of CFUC in S-phase were observed. These data suggest that indomethacin produced an hyperplasia of the committed stem cell compartment. The decrease of PGE2 amounts in bone marrow cells following treatment with indomethacin could explain the hyperplasia observed. These in vivo results are in accordance with the in vitro data which show that PG could control the proliferation and differentiation of myeloid progenitor cells.
通过评估以下指标，研究了吲哚美辛（每天口服6mg/kg，持续2、3或4天）对小鼠骨髓生成的影响：1）骨髓细胞分类计数；2）在粒细胞-巨噬细胞集落刺激因子（GM-CSF）存在的情况下集落形成单位（CFUC）的增殖；3）氚标记胸腺嘧啶核苷自杀后CFUC的增殖状态。治疗4天后，可识别的髓系前体细胞增加，观察到集落形成能力和S期CFUC数量均增加。这些数据表明，吲哚美辛导致定向干细胞区室增生。吲哚美辛治疗后骨髓细胞中前列腺素E2（PGE2）含量的降低可以解释所观察到的增生现象。这些体内结果与体外数据一致，体外数据表明前列腺素可以控制髓系祖细胞的增殖和分化。
