Senegas-Balas F, Balas D, Bouisson M, Ribet A
Digestion. 1981;21(2):83-91. doi: 10.1159/000198546.
Hamster intestinal hydrolase activities were studied after pancreatic duct ligation for periods of 5, 7, 10, 15 and 30 days. From the 7th to the 10th day, maltase and sucrase were significantly increased in the jejunoileum. Higher levels were observed on day 7 in the duodenum for all the brush-border enzyme activities (maltase, sucrase, aminopeptidase, alkaline phosphatase). Intestinal lysozyme significantly increased from the 5th to the 15th day with a maximal level at the 7th day. The increased levels of brush-border enzymes observed here are not in accordance with our description of villous atrophy after pancreatic duct ligation in the hamster. On the other hand, the important increase in lysozyme activity is in good agreement with hypertrophy and hyperplasia of the Paneth cells which we observed during our morphological study. The morphological and biochemical findings on hamster small intestine confirm the effects of exocrine pancreatic secretion both on differentiation and on enzymatic levels of the mucosa. Besides, this experiment agrees with the direct desorbing action of the pancreatic juice on the brush border and suggests another hypothetical mechanism, still worth being investigated, to explain increased brush-border activities in the duodenum and increased levels of lysozyme in the jejunoileum.
在仓鼠胰管结扎5、7、10、15和30天后，对其肠道水解酶活性进行了研究。从第7天到第10天，空回肠中的麦芽糖酶和蔗糖酶显著增加。在第7天，十二指肠中所有刷状缘酶活性（麦芽糖酶、蔗糖酶、氨肽酶、碱性磷酸酶）均观察到较高水平。肠道溶菌酶从第5天到第15天显著增加，在第7天达到最高水平。此处观察到的刷状缘酶水平升高与我们之前对仓鼠胰管结扎后绒毛萎缩的描述不一致。另一方面，溶菌酶活性的显著增加与我们在形态学研究中观察到的潘氏细胞肥大和增生高度一致。仓鼠小肠的形态学和生化研究结果证实了胰腺外分泌对小肠黏膜分化和酶水平的影响。此外，该实验支持胰液对刷状缘的直接解吸附作用，并提出了另一种仍值得研究的假设机制，以解释十二指肠中刷状缘活性增加和空回肠中溶菌酶水平升高的现象。
