大鼠潘氏细胞中肠道磷脂酶A2的免疫组织化学定位
Immunohistochemical localization of intestinal phospholipase A2 in rat paneth cells.
作者信息
Senegas-Balas F, Balas D, Verger R, de Caro A, Figarella C, Ferrato F, Lechene P, Bertrand C, Ribet A
出版信息
Histochemistry. 1984;81(6):581-4. doi: 10.1007/BF00489538.
Using the peroxidase-anti-peroxidase (PAP) technique with a specific rabbit anti-swine intestinal-phospholipase-A2 serum, the immunoreactivity of this phospholipase A2 was localized in rat-intestinal Paneth cells. The specific rabbit anti-swine intestinal-phospholipase-A2 serum did not stain the rat-pancreatic acinar cells which were stained by a specific rabbit anti-swine pancreatic-phospholipase-A2 serum. Specific rabbit anti-swine pancreatic-phospholipase-A2 serum did not stain rat-intestinal Paneth cells. Therefore, there is no cross-immunoreactivity between pancreatic and intestinal phospholipases.
使用过氧化物酶-抗过氧化物酶（PAP）技术以及特异性兔抗猪肠磷脂酶A2血清，这种磷脂酶A2的免疫反应性定位于大鼠肠道潘氏细胞。特异性兔抗猪肠磷脂酶A2血清未对大鼠胰腺腺泡细胞染色，而特异性兔抗猪胰腺磷脂酶A2血清可使大鼠胰腺腺泡细胞染色。特异性兔抗猪胰腺磷脂酶A2血清未对大鼠肠道潘氏细胞染色。因此，胰腺和肠道磷脂酶之间不存在交叉免疫反应性。
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