Iqbal Z M, Epstein S S, Krull I S, Goff U, Mills K, Fine D H
IARC Sci Publ. 1980(31):169-82.
Groups of 3-5 Swiss CD1 mice were gavaged with aqueous solutions of sodium nitrite (250 micrograms), followed immediately by morpholine (5 micrograms) or dimethylamine (250 micrograms). At subsequent intervals, up to 30-60 min, mice were frozen, pulverized in liquid nitrogen and extracted with methylene chloride. Volatile nitrosamines were then quantitatively determined using a Thermal Energy Analyzer, interfaced to GC or HPLC. Biosynthesis of both N-nitrosodimethylamine (NDMA) and N-nitrosomorpholine (NMOR) showed a time-dependent increase for 3 and 9 min, respectively, followed by a steady decline. Levels of NDMA and NMOR were generally less than 0.1 ng/g in control mice gavaged with amine or nitrite alone. Zero-time recovery of NDMA and NMOR ranged from 50-70% and 70-90%, respectively. Biosynthesis of NDMA and NMOR was inhibited by prior administration of ascorbic acid, sodium ascorbate or ammonium sulfamate.
将3至5只瑞士CD1小鼠分为一组，用亚硝酸钠水溶液（250微克）灌胃，随后立即给予吗啉（5微克）或二甲胺（250微克）。在随后长达30至60分钟的时间段内，将小鼠冷冻，在液氮中研磨并以二氯甲烷萃取。然后使用与气相色谱仪或高效液相色谱仪相连的热能分析仪对挥发性亚硝胺进行定量测定。N-亚硝基二甲胺（NDMA）和N-亚硝基吗啉（NMOR）的生物合成分别在3分钟和9分钟内呈时间依赖性增加，随后稳定下降。单独用胺或亚硝酸盐灌胃的对照小鼠中，NDMA和NMOR的水平通常低于0.1纳克/克。NDMA和NMOR的零时间回收率分别为50%至70%和70%至90%。预先给予抗坏血酸、抗坏血酸钠或氨基磺酸铵可抑制NDMA和NMOR的生物合成。
