Hokland P, Heron I, Larsen B
Int Arch Allergy Appl Immunol. 1981;65(2):212-9. doi: 10.1159/000232758.
Two basic proteins (methylated human serum albumin, MHSA, and protamine sulphate, PS) were investigated for their modulatory capacities on a panel of human lymphocyte surface markers. MHSA and PS enhanced the spontaneous rosettes with mouse, sheep and horse erythrocytes, but did not affect the number of rosettes with zymosan particles coated with human complement, or with sheep erythrocytes sensitized with rabbit anti-sheep IgG antibody. Whereas the standard sheep (E) rosette test was not influenced by the negatively charged glycoprotein ceruloplasmin, this compound could, however, abolish the MHSA- and PS-induced enhancements of the number of sheep rosettes. By double marker studies it was furthermore demonstrated that a subset of B cells was induced by MHSA to form E rosettes, and that the same agent increased the number of B cells forming mouse rosettes, so that nearly all B cells were made positive for this marker.
研究了两种碱性蛋白（甲基化人血清白蛋白，MHSA，和硫酸鱼精蛋白，PS）对一组人淋巴细胞表面标志物的调节能力。MHSA和PS增强了与小鼠、绵羊和马红细胞的自发玫瑰花结形成，但不影响与包被人补体的酵母聚糖颗粒或用兔抗绵羊IgG抗体致敏的绵羊红细胞形成的玫瑰花结数量。标准的绵羊（E）玫瑰花结试验不受带负电荷的糖蛋白铜蓝蛋白影响，然而，该化合物可消除MHSA和PS诱导的绵羊玫瑰花结数量增加。通过双标记研究进一步证明，MHSA诱导B细胞亚群形成E玫瑰花结，并且该试剂增加了形成小鼠玫瑰花结的B细胞数量，从而使几乎所有B细胞对该标志物呈阳性。
