Hokland P, Ellegaard J, Heron I
Scand J Haematol. 1981 Mar;26(3):187-94. doi: 10.1111/j.1600-0609.1981.tb01646.x.
Rosettes formed between lymphocytes and unsensitized mouse erythrocytes not only detect a subset of normal b-lymphocytes, but furthermore seem to be of considerable importance in the differential diagnosis of various B cell malignancies. From a series of experiments aimed at optimizing this rosette test, we conclude: (1) Erythrocytes from different mouse strains vary in their affinity for lymphocytes; (2) Experimental conditions are less strict when testing chronic lymphatic leukaemia lymphocytes than normal lymphocytes; (3) Treatment with the sulphydryl compound aminoethyl-isothiouronium bromide, which enhances sheep erythrocyte rosette formation, has an opposite effect on mouse rosettes, and (4) Neuraminidase treatment of the lymphocytes is not necessary when optimal experimental conditions are used.
淋巴细胞与未致敏小鼠红细胞形成的玫瑰花结不仅可检测正常B淋巴细胞的一个亚群，而且在各种B细胞恶性肿瘤的鉴别诊断中似乎具有相当重要的意义。通过一系列旨在优化此玫瑰花结试验的实验，我们得出以下结论：(1) 来自不同小鼠品系的红细胞对淋巴细胞的亲和力各不相同；(2) 检测慢性淋巴细胞白血病淋巴细胞时，实验条件不如检测正常淋巴细胞时严格；(3) 巯基化合物氨基乙基异硫脲溴化物可增强绵羊红细胞玫瑰花结的形成，但对小鼠玫瑰花结有相反的作用；(4) 使用最佳实验条件时，无需对淋巴细胞进行神经氨酸酶处理。
