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Reversed-phase supports for th resolution of large denatured protein fragments.

作者信息

Pearson J D, Mahoney W C, Hermodson M A, Regnier F E

出版信息

J Chromatogr. 1981 Mar 27;207(3):325-32. doi: 10.1016/s0021-9673(00)88735-x.

DOI:10.1016/s0021-9673(00)88735-x
PMID:7228938
Abstract

Large pore (much greater than 300 A), spherical partical silica stationary phases possessing either C18 or C8 hydrocarbon ligands out-performed small pore (60-100A), irregular shaped silicas for the purification of large denatured peptides. Since columns 5 cm in length appeared to be as effective in separating peptides as columns 5 times longer, it is likely that large peptides absorb to the matrix rather than partition between the stationary and mobile phases.

摘要

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