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通过反相高效液相色谱法分离溴化氰衍生的胶原蛋白肽。

Separation of collagen cyanogen bromide-derived peptides by reversed-phase high-performance liquid chromatography.

作者信息

van der Rest M, Bennett H P, Solomon S, Glorieux F H

出版信息

Biochem J. 1980 Oct 1;191(1):253-6. doi: 10.1042/bj1910253.

DOI:10.1042/bj1910253
PMID:7470094
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1162205/
Abstract

A new technique for separation of the CNBr cleavage products of collagen is described. It involves the use of a 30 nm pore reversed-phase high-performance liquid chromatography column eluted with a linear gradient of acetonitrile/water containing 0.01 M-heptafluorobutyric acid. The separation of type I, type II and type III CNBr peptides is described. Resolution is particularly good for the low-molecular-weight peptides. The method is fast, quantitative and sensitive, and the complete volatility of the eluent facilitates the recovery of the separated peptides.

摘要

本文描述了一种分离胶原蛋白溴化氰裂解产物的新技术。该技术使用孔径为30nm的反相高效液相色谱柱，以含0.01M七氟丁酸的乙腈/水线性梯度洗脱。文中介绍了I型、II型和III型溴化氰肽段的分离情况。对于低分子量肽段，分离效果尤其良好。该方法快速、定量且灵敏，洗脱液的完全挥发性有助于回收分离得到的肽段。

相似文献

1
Separation of collagen cyanogen bromide-derived peptides by reversed-phase high-performance liquid chromatography.通过反相高效液相色谱法分离溴化氰衍生的胶原蛋白肽。
Biochem J. 1980 Oct 1;191(1):253-6. doi: 10.1042/bj1910253.
2
A comprehensive approach to the study of collagen primary structure based on high-performance liquid chromatography.一种基于高效液相色谱法的胶原蛋白一级结构综合研究方法。
Eur J Biochem. 1982 Jul;125(3):491-6. doi: 10.1111/j.1432-1033.1982.tb06709.x.
3
Separation of cyanogen bromide peptides of collagen by means of high-performance liquid chromatography.通过高效液相色谱法分离胶原蛋白的溴化氰肽段。
J Chromatogr. 1980 Apr 4;190(2):393-400. doi: 10.1016/s0021-9673(00)88244-8.
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Rapid fractionation of collagen chains and peptides by high-performance liquid chromatography.通过高效液相色谱法快速分离胶原蛋白链和肽段。
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Internal amino acid sequencing of proteins by in situ cyanogen bromide cleavage in polyacrylamide gels.通过聚丙烯酰胺凝胶中的原位溴化氰裂解进行蛋白质的内部氨基酸测序
Biochem Biophys Res Commun. 1990 Jan 15;166(1):139-45. doi: 10.1016/0006-291x(90)91922-f.
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Application of Pluronic copolymer liquid crystals for the capillary electrophoretic separation of collagen type I cyanogen bromide fragments.普朗尼克共聚物液晶在毛细管电泳分离I型胶原溴化氰片段中的应用。
J Chromatogr B Biomed Sci Appl. 2000 Feb 28;739(1):109-16. doi: 10.1016/s0378-4347(99)00555-1.
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Isolation and purification of cyanogen bromide-derived peptides of type II collagen directly from tissue (Swarm chondrosarcoma).
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High-performance liquid chromatography of complex mixtures of cyanogen bromide-produced peptides from different proteins.
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HPLC method to characterize cyanogen bromide collagen fractions containing pyridinoline groups.用于表征含吡啶啉基团的溴化氰胶原蛋白组分的高效液相色谱法。
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Characterization of the CNBr peptides generated from the factor B cleavage fragments, Ba and Bb, by molecular exclusion high performance liquid chromatography.
Immunol Commun. 1982;11(1):47-58. doi: 10.3109/08820138209050723.

引用本文的文献

1
Comparison of buffers and detection systems for high-pressure liquid chromatography of peptide mixtures.肽混合物高压液相色谱分析中缓冲液与检测系统的比较
Biochem J. 1981 Oct 1;199(1):43-51. doi: 10.1042/bj1990043.
2
The behaviour of peptides on reverse-phase supports during high-pressure liquid chromatography.高压液相色谱中肽在反相载体上的行为
Biochem J. 1981 Oct 1;199(1):31-41. doi: 10.1042/bj1990031.

本文引用的文献

1
The nature of the intramolecular cross-links in collagen. The separation and characterization of peptides from the cross-link region of rat skin collagen.胶原蛋白分子内交联的性质。大鼠皮肤胶原蛋白交联区域肽段的分离与鉴定。
Biochemistry. 1966 Nov;5(11):3460-73. doi: 10.1021/bi00875a012.
2
Isolation and characterization of the cyanogen bromide peptides from the alpha-1 chain of rat skin collagen.大鼠皮肤胶原蛋白α-1链溴化氰肽段的分离与鉴定
Biochemistry. 1967 Dec;6(12):3771-80. doi: 10.1021/bi00864a022.
3
Characterization of the product formed by renaturation of alpha 1-CB2, a small peptide from collagen.α1-CB2（一种来自胶原蛋白的小肽）复性形成的产物的特性分析。
Biochemistry. 1970 Oct 13;9(21):4129-33. doi: 10.1021/bi00823a015.
4
Equilibrium and kinetic studies of the helix-coil transition in alpha 1-CB2, a small peptide from collagen.α1-CB2（一种来自胶原蛋白的小肽）螺旋-卷曲转变的平衡和动力学研究。
Biochemistry. 1970 Oct 13;9(21):4134-40. doi: 10.1021/bi00823a016.
5
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
6
Analysis of collagen cyanogen bromide peptides using electrophoresis in continuous concave gradient polyacrylamide gels.使用连续凹形梯度聚丙烯酰胺凝胶电泳分析胶原溴化氰肽。
Anal Biochem. 1979 Jan 1;92(1):183-8. doi: 10.1016/0003-2697(79)90642-0.
7
Ordering of cyanogen bromide peptides of type III collagen based on their homology to type I collagen: preservation of sites for crosslink formation during evolution.基于Ⅲ型胶原蛋白与Ⅰ型胶原蛋白的同源性对其溴化氰肽进行排序：进化过程中交联形成位点的保留。
Proc Natl Acad Sci U S A. 1977 Jan;74(1):84-6. doi: 10.1073/pnas.74.1.84.
8
Human collagen 'fingerprints' produced by clostridopeptidase A digestion and high-pressure liquid chromatography.由梭菌肽酶A消化和高压液相色谱法产生的人胶原蛋白“指纹图谱”
Biochem J. 1977 Mar 1;161(3):527-34. doi: 10.1042/bj1610527.
9
The primary structure of collagen.胶原蛋白的一级结构。
Int Rev Connect Tissue Res. 1976;7:1-60. doi: 10.1016/b978-0-12-363707-9.50007-1.
10
The cyanogen bromide peptides of bovine soluble and insoluble collagens. I. Characterization of peptides from soluble type I collagen by sodium dodecylsulphate polyacrylamide gel electrophoresis.牛可溶性和不溶性胶原蛋白的溴化氰肽。I. 用十二烷基硫酸钠聚丙烯酰胺凝胶电泳对I型可溶性胶原蛋白的肽进行表征。
Connect Tissue Res. 1976;4(2):107-16. doi: 10.3109/03008207609152206.