风疹病毒血凝素玫瑰花结的特性分析
Characterisation of rubella virus hemagglutinin rosettes.
作者信息
Trudel M, Marchessault F, Payment P
出版信息
J Virol Methods. 1981 Mar;2(4):195-201. doi: 10.1016/0166-0934(81)90009-4.
Abstract
Purified rubella virus treated with Triton X-100 was subjected to centrifugation in a sucrose density gradient containing nonionic detergent beta-D-octylglucoside. The result of this treatment was the formation of hemagglutinating rosettes containing viral glycoproteins VP2 (50,000 mol. wt.) and VP3 (63,000 mol. wt.). The rosettes have a 26 S sedimentation coefficient and a density of 1.2 g/cm3 in sucrose. Electron microscopy revealed 15 nm rosettes with a hollow center. The molecular weight of the rosettes was extrapolated at 850,000.
摘要
用Triton X-100处理的纯化风疹病毒在含有非离子去污剂β-D-辛基葡糖苷的蔗糖密度梯度中进行离心。该处理的结果是形成了含有病毒糖蛋白VP2（分子量50,000）和VP3（分子量63,000）的血凝玫瑰花结。这些玫瑰花结在蔗糖中的沉降系数为26 S，密度为1.2 g/cm³。电子显微镜显示中心中空的15 nm玫瑰花结。玫瑰花结的分子量推断为850,000。
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