与在蔗糖、甲泛葡胺和泛影葡胺中进行梯度离心相比，通过琼脂糖2B凝胶过滤法纯化传染性风疹病毒。
Purification of infectious rubella virus by gel filtration on sepharose 2B compared to gradient centrifugation in sucrose, sodium metrizoate and metrizamide.
作者信息
Trudel M, Marchessault F, Payment P
出版信息
J Virol Methods. 1981 Feb;2(3):141-8. doi: 10.1016/0166-0934(81)90032-x.
Rubella virus was purified by chromatography on Sepharose 2B after concentration by ultrafiltration on hollow fibers and hydroextraction with PEG 20,000. Yields of 40% infectivity and 70% hemagglutinating activity were routinely obtained. Chromatographic purification was compared to ultracentrifugation in sucrose, metrizamide and sodium metrizoate. Yields were lower in sucrose and metrizamide, while sodium metrizoate reduced the infectivity of the virus below detectable levels. These results demonstrate the advantage of Sepharose 2B for the purification of infectious rubella virus.
风疹病毒经中空纤维超滤浓缩及用聚乙二醇20,000水抽提后，通过在琼脂糖凝胶2B上进行层析来纯化。通常可获得40%的感染性产量和70%的血凝活性。将层析纯化与在蔗糖、甲泛影酰胺和泛影酸钠中的超速离心进行了比较。在蔗糖和甲泛影酰胺中的产量较低，而泛影酸钠使病毒的感染性降低至检测水平以下。这些结果证明了琼脂糖凝胶2B在纯化感染性风疹病毒方面的优势。
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