Popiel I, Erasmus D A
J Helminthol. 1981 Mar;55(1):33-7.
The rate of uptake of H3 tyrosine by unisexual females in vitro was accelerated by 22% when male worm extract was added to the culture medium. Pairing of unisexual females with mature male worms in vitro resulted in a similar acceleration of H3 tyrosine uptake. As tyrosine is avidly taken up by the vitelline gland for the formation of egg shell protein the change in the rate of H3 tyrosine uptake can be employed as a sensitive quantitative criterion for assessing its development following stimulation.
当向培养基中添加雄虫提取物时,体外培养的孤雌生殖雌虫对H3酪氨酸的摄取速率加快了22%。体外将孤雌生殖雌虫与成熟雄虫配对,也导致H3酪氨酸摄取出现类似的加快情况。由于酪氨酸被卵黄腺大量摄取用于形成卵壳蛋白,因此H3酪氨酸摄取速率的变化可作为评估其受刺激后发育情况的敏感定量标准。