Den Hollander J E, Erasmus D A
Parasitology. 1985 Dec;91 ( Pt 3):449-57. doi: 10.1017/s0031182000062697.
Unisexual females, (80-100 days post-infection) were cultured in vitro and exposed to mature male worms from either mixed or single-sex infections. A comparison was made of the uptake of [3H]thymidine (used as a marker for DNA synthesis) by isolated unisexual females or by those females exposed to males derived from either mixed or unisexual infections during in vitro culture. All males were observed to be paired with females by approximately 18 h post-culture. At 24 h post-culture females paired with males from mixed infections had an elevated uptake of [3H]thymidine compared to females that had not paired and uptake was also significantly different (P less than or equal to 0.001) from females exposed to males from unisexual infections. By 48 h there was no significant difference between uptake in females paired with either type of male. The frequency of uptake in the paired females was approximately double that in unstimulated females while non-paired females in the presence of males had an increase of between 35 and 45%. In a second experiment the effect of depriving a mature egg-laying female of the paired male was investigated. These females had a continual decrease in uptake of [3H]thymidine compared to mature females maintained with male partners. The uptake of [3H]thymidine was used as a marker for DNA synthesis and the effect of the male worm on DNA synthesis in the unisexual female is discussed in view of the above results.
将感染后80 - 100天的孤雌生殖雌性虫体进行体外培养，并使其接触来自混合感染或单性感染的成熟雄虫。对分离出的孤雌生殖雌性虫体，或对那些在体外培养期间接触来自混合感染或单性感染雄虫的雌性虫体，摄取[3H]胸苷（用作DNA合成的标记物）的情况进行了比较。培养后约18小时观察到所有雄虫均与雌虫配对。培养24小时后，与来自混合感染雄虫配对的雌虫对[3H]胸苷的摄取量高于未配对的雌虫，且摄取量与接触来自单性感染雄虫的雌虫也有显著差异（P小于或等于0.001）。到48小时时，与任一类型雄虫配对的雌虫摄取量之间无显著差异。配对雌虫的摄取频率约为未受刺激雌虫的两倍，而在有雄虫存在的情况下未配对雌虫的摄取量增加了35%至45%。在第二个实验中，研究了剥夺成熟产卵雌虫配对雄虫的影响。与有雄虫配偶的成熟雌虫相比，这些雌虫对[3H]胸苷的摄取持续减少。鉴于上述结果，讨论了[3H]胸苷的摄取作为DNA合成标记物以及雄虫对孤雌生殖雌虫DNA合成的影响。
