Scharonowa I N, Chaspekow L G
J Hirnforsch. 1980;21(6):609-25.
The cytoarchitectonic structures, fiber pathways, spontaneous and evoked neuronal activity in the organotypic explants of the newborn mouse hippocampus were studied. After 2-3 weeks in vitro it was possible to see in the explants main fascicles (alvear tract, perforant path, axons of granular cells) and cytoarchitectonic formations (pyramidal cell layer of hippocampus, infra- und suprapyramidal limbs of dentate gyrus granular cells). Electrical stimuli applied to the entorhinal cortex and fascia dentata evoked short- and long-latency responses in hippocampal neurons beginning from the 5-6th day in vitro. The short-latency responses were presumably monosynaptic, the long-lasting ones, polysnaptic. The latter were usually changed by repetitive stimulation. These data suggest that appearance of evoked activity during cultivation reflects the formation in vitro of the perforant path and mossy fibers and the maturation of the synaptic contacts. Thus, organotypic explants of the hippocampus represent a useful model system for morphological and electrophysiological studies in systems of interneuronal connections formed de novo as a result of autonomical postnatal development in vitro.
对新生小鼠海马体的器官型外植体中的细胞构筑结构、纤维通路、自发和诱发的神经元活动进行了研究。在体外培养2 - 3周后，在外植体中可以看到主要的束（海马槽、穿通通路、颗粒细胞轴突）和细胞构筑结构（海马体的锥体细胞层、齿状回颗粒细胞的内、上锥体肢）。从体外培养的第5 - 6天开始，施加于内嗅皮质和齿状筋膜的电刺激在海马神经元中诱发了短潜伏期和长潜伏期反应。短潜伏期反应可能是单突触的，而长潜伏期反应是多突触的。后者通常会因重复刺激而改变。这些数据表明，培养过程中诱发活动的出现反映了体外穿通通路和苔藓纤维的形成以及突触联系的成熟。因此，海马体的器官型外植体代表了一个有用的模型系统，用于研究由于体外自主产后发育而重新形成的神经元间连接系统的形态学和电生理学。
