Widder K J, Senyei A E, Ovadia H, Paterson P Y
J Pharm Sci. 1981 Apr;70(4):387-9. doi: 10.1002/jps.2600700411.
Protein A, a protein derived from Staphylococcus aureus, was incorporated into the matrix of magnetic albumin microspheres. Because staphylococcal protein A binds most subclasses of immunoglobulin G through their Fc portions, immunoglobulin may be rapidly bound to microspheres without chemical coupling agents or a diamino-heptane spacer group. Microspheres so prepared bind specifically to a given cell type when incubated in vitro with a heterogeneous cell population. The use of these microspheres as a drug carrier capable of cellular specificity as well as their ability to isolate homogeneous cell populations rapidly is discussed.
蛋白A是一种源自金黄色葡萄球菌的蛋白质，被整合到磁性白蛋白微球的基质中。由于葡萄球菌蛋白A通过其Fc部分与大多数免疫球蛋白G亚类结合，因此无需化学偶联剂或二氨基庚烷间隔基团，免疫球蛋白就可迅速与微球结合。如此制备的微球在体外与异质细胞群体一起孵育时，能特异性地结合特定的细胞类型。本文讨论了这些微球作为具有细胞特异性的药物载体的用途，以及它们快速分离同质细胞群体的能力。
