一种用于连续测量大鼠肝脏色素排泄的流动池法。

A flow-cell method for measuring hepatic excretion of pigments continuously in rats.

作者信息

Ramonas L M, McDonagh A F, Palma L A

出版信息

J Pharmacol Methods. 1981 Mar;5(2):149-64. doi: 10.1016/0160-5402(81)90007-3.

Abstract

The electronic absorption of effluent bile from restrained rats with indwelling bile cannulas can be measured directly and continuously in the wavelength region 430-800 nm using commercially available micro flow-cells. The method is useful for studying and characterizing the hepatic excretion of endogenous and exogenous pigments. Biliary excretion curves are obtained directly without requiring separate analyses of individual bile samples. To demonstrate its application and scope, the technique has been used to follow the biliary excretion of sulfobromophthalein, rose bengal, biliverdin, and bilirubin.

摘要

使用市售的微流动池，可以直接且连续地测量留置胆汁插管的束缚大鼠流出胆汁在430 - 800 nm波长范围内的电子吸收。该方法对于研究和表征内源性和外源性色素的肝脏排泄很有用。无需对单个胆汁样本进行单独分析即可直接获得胆汁排泄曲线。为了证明其应用和范围，该技术已用于跟踪磺溴酞、孟加拉玫瑰红、胆绿素和胆红素的胆汁排泄情况。