Larrue J, Razaka G, Daret D, Demond J, Bricaud H
Paroi Arterielle. 1980;6(2):85-95.
The elaboration of the extracellular matrix was investigated by radio-autoradiography after incubation of aortic strips, isolated from thoracic aortas of healthy and atherosclerotic rabbits, with tritium labeled proline. Time course experiments indicated that there was a delay of about one hour before significant amounts (25%) of tritiated macromolecules were released from the intact smooth muscle cells. On the contrary, both, the level of incorporation and the rate of excretion of macromolecular components by modified smooth muscle cells of the atherosclerotic area from injured strips were increased. The results indicate that, under the conditions of incubation in vitro, the modified smooth muscle cells of atherosclerotic plaque exhibit a particular behaviour for both, quantitative and qualitative features of macro-molecular synthesis of the extracellular matrix.
用氚标记的脯氨酸孵育从健康和动脉粥样硬化兔胸主动脉分离的主动脉条后，通过放射自显影研究细胞外基质的形成。时间进程实验表明，在大量（25%）的氚标记大分子从完整的平滑肌细胞释放之前，大约有一小时的延迟。相反，损伤条带的动脉粥样硬化区域的修饰平滑肌细胞对大分子成分的掺入水平和排泄速率均增加。结果表明，在体外孵育条件下，动脉粥样硬化斑块的修饰平滑肌细胞在细胞外基质大分子合成的定量和定性特征方面均表现出特殊行为。
