多胺与DNA的相互作用：一项23Na核磁共振研究。
The interaction of polyamines with DNA: a 23Na NMR study.
作者信息
Burton D R, Forsén S, Reimarsson P
出版信息
Nucleic Acids Res. 1981 Mar 11;9(5):1219-28. doi: 10.1093/nar/9.5.1219.
Abstract
The interaction between a variety of polyamines, both naturally occurring and synthetic, and calf thymus DNA has been studied using 23Na NMR. The relaxation behaviour of 23Na reflects the extent of interaction of Na+ with DNA phosphate groups and therefore the extent of charge neutralisation of DNA phosphate groups (P) by polyamine amino and imino groups (N) in solutions of DNa, polyamine and Na+. The studies reveal that whereas spermine and spermidine are capable of expelling nearly all of the Na+ ions from DNA at N/P approximately 1, diamines such as putrescine and homologues of spermine and spermidine are capable of neutralising only roughly 50% of DNA phosphates. The results provide a challenge to current models of DNA-polyamine interactions.
摘要
利用23Na核磁共振研究了多种天然存在和合成的多胺与小牛胸腺DNA之间的相互作用。23Na的弛豫行为反映了Na+与DNA磷酸基团的相互作用程度，因此也反映了在DNA、多胺和Na+溶液中，多胺的氨基和亚氨基（N）对DNA磷酸基团（P）的电荷中和程度。研究表明，在N/P约为1时，精胺和亚精胺能够将几乎所有的Na+离子从DNA中排出，而诸如腐胺以及精胺和亚精胺同系物等二胺只能中和大约50%的DNA磷酸盐。这些结果对当前的DNA - 多胺相互作用模型提出了挑战。
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