Wandel M, Norum K R, Berg T, Ose L
Scand J Gastroenterol. 1981;16(1):71-80.
Binding, uptake, and degradation of 125I-labelled HDL were measured in isolated rat non-parenchymal cells in vitro. The binding exhibited saturation kinetics and was inhibited to various degrees with other unlabelled lipoproteins such as VLDL, LDL, and HDL, but the uptake was not reduced by heat and formaldehyde-denatured albumin. The binding could be reduced by pronase treatment of the cells. Acetylated 125I-labelled HDL were more effectively taken up and degraded by non-parenchymal cells than unmodified 125I-labelled HDL. The Kupffer cells were able to take up two to three times as much HDL per cell as the endothelial cells. 125I-labelled HDL bind to the plasma cell membrane and can be dissociated by addition of unlabelled HDL. Relatively more labelled HDL dissociate at 4 degrees C than at 37 degrees C. The non-parenchymal cells also internalize 125I-labelled HDL at 37 degrees C, and the lysosomotropic drug chloroquine inhibits partly the degradation of accumulated 125I-labelled HDL.
在体外分离的大鼠非实质细胞中测量了¹²⁵I标记的高密度脂蛋白（HDL）的结合、摄取和降解。结合表现出饱和动力学，并且受到其他未标记的脂蛋白（如极低密度脂蛋白、低密度脂蛋白和高密度脂蛋白）不同程度的抑制，但摄取不受热和甲醛变性白蛋白的影响。细胞经链霉蛋白酶处理后，结合能力会降低。与未修饰的¹²⁵I标记的HDL相比，乙酰化的¹²⁵I标记的HDL更有效地被非实质细胞摄取和降解。枯否细胞每个细胞摄取HDL的量是内皮细胞的两到三倍。¹²⁵I标记的HDL与质膜结合，添加未标记的HDL可使其解离。在4℃时比在37℃时有相对更多的标记HDL解离。非实质细胞在37℃时也会内化¹²⁵I标记的HDL，溶酶体促渗药物氯喹部分抑制积累的¹²⁵I标记的HDL的降解。
