Panin L E, Usynin I F, Poliakov L M
Vopr Med Khim. 1986 Jul-Aug;32(4):106-10.
Individual hepatocytes and sinusoid cells were isolated from rat liver tissue within various periods after intravenous administration of 125I-lipoproteins of very low (VLDL), low (LDL) and high density (HDL). Estimation of the radioactivity per 1 mg of the cell protein showed that within 30 min absorption of VLDL was 6-fold, LDL--4-fold and HDL--14-fold as high as compared with hepatocytes. Within the first 30 min sinusoid cells accumulated 54% of 125I-HDL, while hepatocytes contained 46% of the labelled lipoprotein, absorbed in liver tissue. On the other hand, within 6 hrs and 12 hrs hepatocytes contained 80-88% and sinusoid cells--17-20% of the label. After fractionation of hepatocytes in Ficoll density gradient all the populations of hepatocytes were shown to absorb effectively VLDL fraction, while HDL and LDL were accumulated in "heavy" (centrolobular) hepatocytes. Penetration of the lipoproteins in hepatocytes appears to be carried out by means of intracellular transport from sinusoid cells.
在静脉注射极低密度脂蛋白（VLDL）、低密度脂蛋白（LDL）和高密度脂蛋白（HDL）的125I标记脂蛋白后的不同时间段，从大鼠肝脏组织中分离出单个肝细胞和窦状隙细胞。每毫克细胞蛋白的放射性测定显示，与肝细胞相比，30分钟内VLDL的吸收量高6倍，LDL高4倍，HDL高14倍。在最初的30分钟内，窦状隙细胞积累了肝脏组织中吸收的125I-HDL的54%，而肝细胞含有46%的标记脂蛋白。另一方面，6小时和12小时后，肝细胞含有80-88%的标记物，窦状隙细胞含有17-20%。在Ficoll密度梯度中对肝细胞进行分级分离后，所有肝细胞群体均显示能有效吸收VLDL组分，而HDL和LDL则积累在“重”（中央小叶）肝细胞中。脂蛋白进入肝细胞似乎是通过从窦状隙细胞进行细胞内转运来实现的。
