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来自牛支原体的炎性毒素:分离与特性鉴定

Inflammatory toxin from Mycoplasma bovis: isolation and characterization.

作者信息

Geary S J, Tourtellotte M E, Cameron J A

出版信息

Science. 1981 May 29;212(4498):1032-3. doi: 10.1126/science.7233196.

DOI:10.1126/science.7233196
PMID:7233196
Abstract

An inflammatory toxin was extracted from Mycoplasma bovis with 75 percent aqueous ethanol. The toxin is a complex polysaccharide composed of glucose, glucosamine or galactosamine, and a heptose, is heat-stable, devoid of protein and lipid, and has a molecular weight of 73,000. The holotoxin in the cell membrane is a glycoprotein; however, it is the polysaccharide portion that is toxic. This inflammatory toxin increases vascular permeability and is capable of activating complement. Infusion of 0.9 milligram of toxin into the bovine udder resulted in the characteristic eosinophilic mastitis produced by Mycoplasma bovine.

摘要

用75%的乙醇水溶液从牛支原体中提取出一种炎性毒素。该毒素是一种由葡萄糖、氨基葡萄糖或半乳糖胺以及一种庚糖组成的复合多糖,热稳定,不含蛋白质和脂质,分子量为73000。细胞膜中的全毒素是一种糖蛋白;然而,有毒的是多糖部分。这种炎性毒素可增加血管通透性并能够激活补体。向奶牛乳房内注入0.9毫克毒素会导致由牛支原体引起的典型嗜酸性乳腺炎。

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