Hoffman R A, Ascher N L, Nelson R D, Simmons R L
Transplantation. 1981 Mar;31(3):195-200. doi: 10.1097/00007890-198103000-00011.
Normal unstimulated mouse lymph node lymphocytes (LNLs) migrated into filters in a gradient of normal mouse serum (NMS), heat-inactivated mouse serum (HI-MS), or zymosan-activated mouse serum (ZAS). Blind well chemotaxis chambers with 5-micrometer pore size cellulose nitrate membranes were used. Migration was assessed both by the leading front technique and the mean aggregate number. A concentration of 2.5 x 10(6) LNLs/ml or greater was needed to detect migration. Migration of LNLs to 1% NMS was time dependent and was inhibited by cytochalasin B. Comparison of the migration patterns of LNLs, neutrophils, and macrophages revealed that all cell types were responsive to NMS. LNLs responded as well to HI-MS as they did to NMS, neutrophils responded less well to HI-MS than to MMS, and macrophages did not respond to HI-MS. The LNL response to ZAS was significantly greater than the response to NMS to HI-MS and neutrophils and macrophages also responded strongly to ZAS. The migration of LNLs from various mouse strains to NMS revealed that the LNLs from different mouse strains possess varying degree of motility. The factor in mouse serum which induced migration was not strain specific. The LNLs from peripheral (inguinal, axillary, and brachial) nodes demonstrated greater motility in response to NMS than mesenteric LNLs. Using the checkerboard assay to discriminate chemotaxis from chemokinesis, mouse serum appeared to be solely chemokinetic when the leading front technique was used. However, using the mean aggregate number technique, mouse serum was determined to be both chemokinetic acid chemotactic for LNLs. The results indicate that the method can be reliably used to study those factors which influence the motility of normal or altered populations of lymphocytes.
正常未受刺激的小鼠淋巴结淋巴细胞（LNLs）在正常小鼠血清（NMS）、热灭活小鼠血清（HI-MS）或酵母聚糖激活的小鼠血清（ZAS）梯度中迁移到滤膜上。使用带有5微米孔径硝酸纤维素膜的盲孔趋化性小室。通过前沿技术和平均聚集数来评估迁移情况。需要2.5×10⁶个LNLs/ml或更高的浓度才能检测到迁移。LNLs向1%NMS的迁移是时间依赖性的，并受到细胞松弛素B的抑制。对LNLs、中性粒细胞和巨噬细胞迁移模式的比较表明，所有细胞类型都对NMS有反应。LNLs对HI-MS的反应与对NMS的反应相同，中性粒细胞对HI-MS的反应比对MMS的反应差，而巨噬细胞对HI-MS无反应。LNLs对ZAS的反应明显大于对NMS或HI-MS的反应，中性粒细胞和巨噬细胞对ZAS也有强烈反应。来自不同小鼠品系的LNLs向NMS的迁移表明，不同小鼠品系的LNLs具有不同程度的运动性。小鼠血清中诱导迁移的因子不是品系特异性的。来自外周（腹股沟、腋窝和臂部）淋巴结的LNLs对NMS的反应比肠系膜LNLs表现出更大的运动性。使用棋盘分析来区分趋化作用和化学增活作用，当使用前沿技术时，小鼠血清似乎仅具有化学增活作用。然而，使用平均聚集数技术时，小鼠血清被确定对LNLs既有化学增活作用又有趋化作用。结果表明，该方法可可靠地用于研究影响正常或改变的淋巴细胞群体运动性的那些因素。
