Lawson B M, Valli V E, Mills J N, Forsberg C M
Tropenmed Parasitol. 1980 Dec;31(4):425-34.
Myeloid and erythroid cultures were prepared from bone marrow aspirates taken from control and T. congolense infected neonatal and six-month-old calves. The aspirates obtained from the infected animals were more cellular than the controls and it was found that the removal of adherent cells prior to Ficoll separation improved the sensitivity of the cultures to colony stimulating factors and removed the background connective tissue cells and megakaryocytes from the resulting colonies. There was an apparent decrease in the number of myeloid colonies from the infected six-month-old calves, but an initial increase in myeloid colonies in the infected neonatal calves compared to their controls. There were no significant differences between infected and control calves for the numbers of erythroid colonies produced, but the degree of maturation as measured by hemoglobinization was decreased in two infected six-month-old calves at week 13 of infection.
从对照以及感染刚果锥虫的新生和6月龄小牛的骨髓穿刺物中制备髓系和红系培养物。从感染动物获得的穿刺物比对照的细胞更多，并且发现，在Ficoll分离之前去除贴壁细胞可提高培养物对集落刺激因子的敏感性，并从所得集落中去除背景结缔组织细胞和巨核细胞。感染的6月龄小牛的髓系集落数量明显减少，但与对照相比，感染的新生小牛的髓系集落在初期有所增加。感染小牛和对照小牛产生的红系集落数量没有显著差异，但在感染第13周时，两只感染的6月龄小牛中，通过血红蛋白化测量的成熟度有所降低。
