Mills J N, Valli V E, Boo K S, Forsberg C M
Tropenmed Parasitol. 1980 Sep;31(3):299-312.
Hemocytometer and cytofluorometric methods were used to count trypanosomes in the blood of neonatal and six-month-old calves infected with T. congolense. The correlation between these methods was highest in the six-month-old calves because their parasitemia was greater and the accuracy of cytofluorometric counting is reduced below 1,000 trypanosomes per microliter of blood. Fine needle aspiration biopsies showed many trypanosomes marginated in cerebral cortical capillaries, but few in muscle or liver. Quantitatively, the cerebral capillaries in the infected six-month-old calves were significantly wider than their controls with fewer endothelial nuclei suggesting capillary dilatation. There were fewer trypanosomes and no significant changes in capillary width in the neonatal calves. Several drugs were tried for activity in dispersing marginated trypanosomes into axial flow. Berenil and dexamethasone had a significant effect on trypanosome counts in blood and tissue whereas, epinephrine caused a fleeting increase in parasitemia. Hetastarch, dextran and cyclophosphamide had no specific effect. Fine needle aspiration bipsies of brain, thirty minutes after Berenil injection showed that the capillaries were cleared of trypanosomes.
使用血细胞计数器和细胞荧光测定法对感染刚果锥虫的新生犊牛和6月龄犊牛血液中的锥虫进行计数。在6月龄犊牛中，这两种方法之间的相关性最高，因为它们的寄生虫血症更高，并且当每微升血液中的锥虫数量低于1000个时，细胞荧光测定计数的准确性会降低。细针穿刺活检显示，许多锥虫位于大脑皮质毛细血管边缘，但在肌肉或肝脏中较少。定量分析表明，感染的6月龄犊牛的脑毛细血管明显比对照宽，内皮细胞核较少，提示毛细血管扩张。新生犊牛中的锥虫较少，毛细血管宽度也无显著变化。尝试了几种药物来观察其将边缘锥虫分散到轴流中的活性。贝尼尔和地塞米松对血液和组织中的锥虫计数有显著影响，而肾上腺素导致寄生虫血症短暂增加。羟乙基淀粉、右旋糖酐和环磷酰胺没有特定作用。注射贝尼尔30分钟后对大脑进行细针穿刺活检显示，毛细血管中的锥虫已清除。
