Brenner R J, Cupp E W, Bernardo M J
Tropenmed Parasitol. 1980 Dec;31(4):487-97.
Colonization techniques were developed in order to select mating strains from four geographic populations of Simulium decorum. Although mating strains were established from each locale, emphasis was shifted toward developing permanent colonies from Dryden Lake, New York, USA and Warm Springs, Georgia, USA. The net reproductive rate (Ro) was computed for the parental, F1, and F2 generations of each strain in order to assess the effects of colonization through successive generations. Ro increased rapidly for both strains from parental (range of 0.34-3.5) through F2 generations (range of 16.2-21.6). Analysis of Ro components indicated that the increases in Ro were the result of two parameters that reflect the propensity of flies to mate in confinement, suggesting that selection for mating is rapid. Multiple regression analysis indicated no significant differences between strains or generations when tested against fecundity (range of 391-519) and survival of immatures (range of 90.3-97.2%). A slight positive correlation (regression coefficient of 0.0016, P less than .01) was observed between larval density (range of 284-4,439 larvae per rearing) and survival of immatures, suggesting that the limits of the rearing system with regard to crowding were not approached. Both strains readily adapt to the laboratory and are currently in the fifth generation.
为了从装饰蚋（Simulium decorum）的四个地理种群中选择交配品系，开发了定殖技术。尽管从每个地点都建立了交配品系，但重点转向了从美国纽约州德莱顿湖和美国佐治亚州温泉建立永久性种群。计算了每个品系亲代、F1和F2代的净生殖率（Ro），以评估连续几代定殖的影响。两个品系的Ro从亲代（范围为0.34 - 3.5）到F2代（范围为16.2 - 21.6）都迅速增加。对Ro组成部分的分析表明，Ro的增加是两个反映果蝇在有限空间内交配倾向参数的结果，这表明对交配的选择很快。多元回归分析表明，在针对繁殖力（范围为391 - 519）和未成熟个体存活率（范围为90.3 - 97.2%）进行测试时，品系或代之间没有显著差异。在幼虫密度（每个饲养环境中幼虫范围为284 - 4439只）和未成熟个体存活率之间观察到轻微的正相关（回归系数为0.0016，P小于0.01），这表明饲养系统在拥挤方面尚未达到极限。两个品系都很容易适应实验室环境，目前已到第五代。
